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70. Phospholipid arrays on porous polymer coatings generated by micro-contact spotting

 

Sylwia Sekula-Neuner, Monica de Freitas, Lea-Marie Tröster, Tobias Jochum, Pavel A Levkin, Michael Hirtz, and Harald Fuchs

Beilstein J Nanotechnol. 2017; 8: 715–722.
 doi: 10.3762/bjnano.8.75,

 

 
 
Nanoporous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) (HEMA-EDMA) is used as a 3D mesh for spotting lipid arrays. Its porous structure is an ideal matrix for lipid ink to infiltrate, resulting in higher fluorescent signal intensity as compared to similar arrays on strictly 2D substrates like glass. The embedded lipid arrays show high stability against washing steps, while still being accessible for protein and antibody binding.


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